THIAMINE PYROPHOSPHOKINASE ACTIVITY IN LIVER, HEART AND BRAIN CRUDE EXTRACTS OF CONTROL AND THIAMINE-DEFICIENT RATS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 47  (4) , 315-324
Abstract
The activity of thiamine pyrophosphokinase (EC 2.7.6.2) was measured in liver, heart muscle and brain of normal and thiamine deficient rats. The activity measurement was performed by use of thiamine-35S as substrate and separation of the reaction products by high voltage electrophoresis. KM was determined as 7.1 .cntdot. 10-6 mol/l. The activity of thiamine pyrophosphokinase is reduced in liver and heart muscle of thiamine deficient rats significantly, but no decrease of the enzyme activity was found in the brain. The content of thiamine pyrophosphate was measured in the liver of normal and thiamine deficient rats. Injection of thiamine to deficient rats normalized the content of thiamine pyrophosphate in the liver within 6 h. Apparently thiamine pyrophosphokinase is an adaptive enzyme, the activity of which depends on the thiamine content of the cells.

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