Tracking bacterial DNA replication forksin vivoby pulsed field gel electrophoresis
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (9) , 3479-3490
- https://doi.org/10.1093/nar/17.9.3479
Abstract
The location of chromosomal DNA replication forks was identified in synhronously replicating Escherichia coli cultures by pulse labeling DNA at specific times with 14C-thymidine and following incorporation of radionucleotide into genomic Not I restriction fragments. This technique could be used to characterize chromosomal DNA replication, to characterize mutations which affect this process, to identify the location of DNA replication origins and termini as well as aid in the construction of macrorestriction maps. Here, we further characterize the DNA replication mutations divE and dnaK and preliminarily characterize the genomic organization E. coli isolate 15.This publication has 39 references indexed in Scilit:
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