Characterization of fractionated polyclonal antibodies for immunoaffinity chromatography
- 1 May 1988
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 31 (7) , 635-642
- https://doi.org/10.1002/bit.260310702
Abstract
Polyclonal anti‐BSA antibodies were ractionated by stepwise elution from an immobilized BSA column by decreasing pH or increasing the concentration of NaSCN. The binding affinities of each fraction and original globulin under physiological conditions and their dependence on pH and ionic environments were compared. Fractions with high association constant under physiological conditions did not necessarily show antigen binding affinity over a wide pH range, but they retained a high affinity at higher ionic strength of NaSCN. Consequently, by combining these two fractionation procedures, a fraction with high affinity and which dissociated at moderate pH was obtained. It is clear that high affinity is not always incompatible with ease of dissociation accompanying a change in conditions.Keywords
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