Nuclear assembly is independent of linker histones.
- 20 December 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (26) , 12477-12481
- https://doi.org/10.1073/pnas.91.26.12477
Abstract
The role of linker histones in the assembly of functional nuclei was examined with the use of a cell-free extract of Xenopus eggs that transforms condensed sperm chromatin into DNA-replication-competent pronuclei. When linker histones were removed from the extract, the resultant pronuclei were indistinguishable from those formed in the complete extract. The assembly of functional nuclear membrane, nuclear lamina, and prereplication centers allowed identical DNA replication efficiencies. Thus, linker histones are not required for the assembly of morphologically normal nuclei capable of DNA replication.Keywords
This publication has 31 references indexed in Scilit:
- A role for histones H2A/H2B in chromatin folding and transcriptional repression.Proceedings of the National Academy of Sciences, 1994
- The Maternal Histone H1 Variant, H1M (B4 Protein), Is the Predominant H1 Histone in Xenopus Pregastrula EmbryosDevelopmental Biology, 1994
- Four distinct and unusual linker proteins in a mitotically dividing nucleus are derived from a 71-kilodalton polyprotein, lack p34cdc2 sites, and contain protein kinase A sites.Molecular and Cellular Biology, 1994
- Chromosome Condensation in Xenopus Mitotic Extracts Without Histone H1Science, 1993
- Chromatin Transitions during Early Xenopus Embryogenesis: Changes in Histone H4 Acetylation and in Linker Histone TypeDevelopmental Biology, 1993
- SAR-dependent mobilization of histone H1 by HMG-I/Y in vitro: HMG-I/Y is enriched in H1-depleted chromatin.1993
- Absence of Somatic Histone H1 in Oocytes and Preblastula Embryos of Xenopus laevisDevelopmental Biology, 1993
- Replication Timing and Xenopus 5S RNA Gene Transcription in VitroDevelopmental Biology, 1993
- Influence of chromatin folding on transcription initiation and elongation by RNA polymerase IIIBiochemistry, 1992
- Chromatin condensation: does histone H1 dephosphorylation play a role?Trends in Biochemical Sciences, 1992