STUDIES ON DENITRIFICATION

Publisher Website
Google Scholar
Abstract
Acetone-dried powder of the cells of Pseudomonas denitrificans was extracted at pH 6.8. The supernatant was fractionated by (NH4SO)2 SO4 at 0.33 0.45 saturation (fraction A) and 0.45 v-0.70 saturation (fraction B). Fractions A and B were active only when combined. After passing fraction A through an Amberlite CG-50 column, and cytochrome c1552 was precipitated by (NH4)2SO4 at 0.60 saturation. Fraction B was subjected to Amberlite CG-50 column chromatography, and upper reddish zone of cryptocyto-chrome c and lower blue protein(s) were eluted with 0.5[image] phosphate buffer, pH 6.8. Cytochrome c1552 showed absorption maxima at 552, 523, 418 mu (reduced form) and 540(weak), 408 m[mu] (oxidized form), while cryptocytochrome at 540-560, 423, 430-440 (shoulder) m[mu] (reduced form), and 500 (weak), 394 m[mu] (oxidized form). Only cryptocytochrome c was reduced toy a mixture of HNO2 and NH2OH, leading to the NO2 formation. Cytochrome c1552 was demonstrated as an electron carrier between H-donor (dimethyl p-phenylenediamine) and HNO2.

This publication has 2 references indexed in Scilit: