Electron-Microprobe Analysis of Isolated Plant Cells
- 1 August 1971
- journal article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 22 (3) , 749-752
- https://doi.org/10.1093/jxb/22.3.749
Abstract
A technique is described for the study of the intracellular localization of minerals in a single isolated plant cell by Electron Microprobe X-ray Analyser. Tissue-culture cells were micro-analysed for zinc, calcium, potassium, and phosphorus. The X-ray oscillograms showed accumulation of zinc, potassium, and phosphorus in the nuclei. Microprobe analysis is a non-destructive method and allows for multiple element analysis in the same tissue, thereby having a wide application in the study of biological materials at cellular and subcellular levels.Keywords
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