Effect of Adenine Nucleotides on Catecholamine Release and Uptake in Isolated Adrenergic Nerve Granules

Abstract
On addition of 1–5 mM adenosine triphosphate (ATP) the spontaneous loss of noradrenaline from a suspension of adrenergic nerve granules is retarded or prevented. Adenosine diphosphate (ADP) exerts a similar effect while adenosine monophosphate (AMP) has little action. ADP and ATP also enhance the uptake of catecholamines from the incubation fluid. This effect is observed even with low amine concentrations in the incubation fluid in contrast to the uptake without addition of ATP. The ATP‐dependent uptake is inhibited by reserpine.