Experimental investigations on the influence upon the chemical carcinogenesis

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Abstract
After ten times monthly, subcutaneous, injections of 30mg/kg 1,2-dimethylhydrazine (DMH) to Sprague-Dawley-rats, malignant tumors were found in ∼90% of the animals after a mean induction period of ∼330 days. Injections were started on the 2nd day of life and maintained during 10 months. 72% of the tumors induced were formed in the colon, 17% were squamous cell carcinomas of the ear duct, adenosarcomas of the kidney and hepatocellular carcinomas were found in 13 and 11% of the animals, respectively. Additional treatment with immunodepressive (cyclophosphamide, methotrexat, hydrocortisone) and immunostimulating substances (BCG, albumin, vitamin A-acid) as well as an enzyme-stimulating agent (Luminal) did not alter incidences and induction periods of tumors. After application of a vegetarian diet, the rate of liver and kidney tumors was diminished significantly and induction periods of intestinal and ear duct tumors increased. Nach zehnmaliger, monatlicher, subcutaner Injektion von 30 mg/kg 1,2-Dimethylhydrazin an Sprague-Dawley-Ratten, beginnend am 2. Lebenstag, entwickelten ∼90% der Tiere nach ∼330 Tagen Adenocarcinome des Dickdarms (72%), Plattenepithelcarcinome des Gehörgangs (17%). Adenosarkome der Niere (13%) sowie hepatocelluläre Carcinome (11%). Zusatzbehandlungen mit Immunodepressiva (Cyclophosphamid, Methotrexat, Hydrocortison), Immunostimulantien (BCG, Albumin, Vitamin A-Säure) oder Enzymstimulatoren (Luminal) änderten die Tumorausbeuten und Induktionszeiten nicht. Nach Applikation einer vegetarischen Diät waren die Ausbeuten an Nieren- und Leberkrebsen signifikant vermindert und die Induktionszeiten der Darm- und Gehörgangskrebse vergrößert.