Immunofluorescent Detection of Factor VIII-Related Antigen in Human Platelets and Megakaryocytes

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Abstract
Platelets, lymphocytes, and megakaryocytes, specifically processed for the intracellular detection of antigens, were studied by indirect immunofluorescence techniques using monospecific antibodies directed against factor VIII-related antigen (F-VIII-RA). Specific staining was observed in platelets of the peripheral blood from normal individuals and from patients with haemophilia A, and in megakaryocytes and platelets of bone marrow from normal subjects. The same cells studied in patients with von Willebrand’s disease were negative. No bone marrow biopsies were performed on patients with haemophilia A. Our results indicate that F-VIII-RA is localized in megakaryocytes and platelets of normal subjects; the possible role of this F-VIII-RA in platelet function is discussed.