AN EVALUATION OF VARIOUS METHODS FOR THE BIOASSAY OF DIHYDROTACHYSTEROL

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Abstract
The present method for the bioassay of dihydrotachysterol is based on detn. of the "toxic borderline dose," according to Holtz, et al. The fact that this is not a specific means of determining the hypercalcemic principle in activated sterols, and the rather poor quantitative reproducibility of the results, have led to an examination of other methods available for this purpose. Detn. of the antirachitic potency of preps. of dihydrotachysterol gives results which are acceptable as to reproducibility but are no more specific for the hypercalcemic principle than the established method. Detn. of the hypercalcemic effect in rats has proved satisfactory as to reproducibility, and is also a specific measure of the active principle. The assay based on hypercalcemic effects in dogs has not proved feasible because of the wide variations in individual reaction to the prep. A very uniform group of dogs would be required for this purpose.

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