A human lymphoid cell line with an IgG‐like membrane component

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Abstract
A human lymphoid cell line (S95) with surface‐located IgG is described. The line was derived from a patient with Hodgkin's disease and is aneuploid. Its characterstics are suggestive of a malignant B lymphocyte derivation, but no ultimate proof of malignancy can be forwarded. The line produces immunoglobulin (IgG) which appears to be devoid of light chain. The amount of IgG detectable at the cell surface by inhibition of the rosette formation by protein A‐coated sheep red blood cells with protein A was 5–10 ng/106 cells corresponding to 70 000 – 140 000 molecules/cell. The immunoglobulin is membrane bound. Partial characterization of the IgG‐like protein shows that it has a molecular weight of 50 000 Daltons and that the molecule lacks some of the normal γ‐chain determinants.