Anti-poly(A) polymerase antibodies in the sera of tumor-bearing rats can inhibit specific cleavage and polyadenylation reactions
- 1 May 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 12 (5) , 917-920
- https://doi.org/10.1093/carcin/12.5.917
Abstract
Sera from rats bearing Morris hepatoma 3924A for 7 weeks contained antibodies against the 48 kd tumor-type poly(A) polymerase, wherease sera from normal rats or rats bearing the tumor for shorter periods did not exhibit immunoreactivity against the enzyme. Purified IgG from the sera of the tumor-bearing rats inhibited both cleavage at the poly(A) site and polyadenylation of adenovirus L3 RNA in nuclear extracts derived from rats did not block the 3′-terminal processing reaction. Control or immune IgG had not efect on the transcription of ribosomal gene in theextracts derived from H-4hepatoma cells. These data demonstrate the functional specificity of the anti-poly(A) polymerase antibodies found in the sera of the tumor-bearing rats.Keywords
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