Comparison of the expression of a T?1:nlacZ transgene and T?1 ?-tubulin mRNA in the mature central nervous system

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Abstract
We have previously demonstrated that one member of the α-tubulin multigene family, termed Tα1 in rats, is a panneuronal gene that is regulated as a function of neuronal growth and regeneration. Moreover, 1.1 kb of the 5′ upstream region from this gene is sufficient to direct expression of a marker gene to growing neurons in transgenic mice. In this report, we have characterized the distribution of the Tα1:nlacZ transgene in the mature central nervous system in two lines of transgenic mice and have compared its expression to that of the endogenous Tα1 α-tubulin mRNA. These results demonstrate that the pattern of expression of the Tα1:nlacZ transgene is similar to that of Tα1 mRNA, with a few notable differences. Furthermore, expression of the transgene and the mRNA within the mature brain is panneuronal and, in many cases, is highest in those populations of neurons that show some capacity for morphological growth. These results, together with our previous studies on mature regenerating neurons (Gloster et al. [1994] J. Neurosci. 14:7319–7330; Wu et al. [1994] Soc. Neurosci. Abstr. 20:542) suggest that the Tα1:nlacZ transgene will provide a useful marker of growth-associated gene expression in the mature nervous system.