Studies on the absorption, distribution, excretion and metabolism of ginseng saponins. I. Quantitative analysis of ginsenoside Rg1 in rats.

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Abstract
Quantitative analysis of ginsenoside Rg1, isolated from red ginseng (Panax ginseng), and 1 of the main saponins was performed in rats to study the absorption, distribution, excretion and metabolism of ginseng saponins. Ginsenoside Rg1 added to biological samples of rats was adsorbed on a Servachrom XAD-2 resin column after deproteinization with methanol. The adsorbed ginsenoside Rg1 was eluted with 60% methanol aqueous solution and further subjected to TLC with CHCl3-CH3OH-H2O (65:35:10, lower phase) as the developing solvent and 8% vanillin methanol solution/72% H2SO4 (1:5) as the detecting reagent. As reasonable recoveries and SD were found in this procedure, the concentrations of ginsenoside Rg1 in samples from rats treated with oral ginsenoside Rg1 (100 mg/kg) were determined by applying this method.