Colorimetric Determination of Allyl Isothiocyanate
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 16 (15) , 1197-1206
- https://doi.org/10.1080/00032718308077156
Abstract
A simple and accurate colorimetric method for determination of allyl isothiocyanate was developed. It involves formation of methylene blue by the reaction of an acid solution of N, N-dimethyl-p-phenylenediamine in the presence of ferric ion with the sulfide liberated by ethanolic base hydrolysis of allyl isothiocyanate in xylene. This method permits determination of microgram quantities of S in allyl isothiocyanate. The effects of alterations in reaction conditions on the color development were investigated. Tests with a range of sulfur-containing organic compounds (thiourea, cysteine, methionine, cysteic acid, thioacetamide, benzene sulfonamide, glucose-6-Sulfate, and sinigrin) showed that none of the compounds tested interfere with this method when the S concentration is ≤ 10 times that of S as allyl isothiocyanate.Keywords
This publication has 5 references indexed in Scilit:
- Role of Microorganisms in the Atmospheric Sulfur CyclePublished by Springer Nature ,1978
- Studies on Fungous MyrosinaseAgricultural and Biological Chemistry, 1969
- Myrosinase Activity in Bacteria as Demonstrated by the Conversion of Progoitrin to Goitrin.Experimental Biology and Medicine, 1965
- A direct microdetermination for sulfideAnalytical Biochemistry, 1965
- A thioglucosidase in fungiArchives of Biochemistry and Biophysics, 1958