The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins
- 1 March 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (3) , 761-770
- https://doi.org/10.1002/bit.260250312
Abstract
The effect of chaotropic salts on the dissociation of ribonucleic acid from yeast nucleoprotein complex was studied. The effectiveness of various salts on the dissociation of ribonucleic acid followed the chaotropic series; i.e., Cl3CCOONa = NaClO4 > NaBr > NaCl. Treatment of the nucleoprotein complex with 0.5M Cl3CCOONa or NaClO4 resulted in RNA removal of about 80%, whereas NaCl and NaBr removed only about 10 and 25%, respectively. Based on the results presented, a simple and novel method for industrial‐scale preparation of single‐cell proteins with low levels of nucleic acid is proposed.This publication has 18 references indexed in Scilit:
- Reversible Modification of Lysine: Separation of Proteins and Nucleic Acids in YeastPublished by American Chemical Society (ACS) ,1982
- Thermodynamics of protein association reactions: forces contributing to stabilityBiochemistry, 1981
- Ribonuclease isolated from yeast (Saccharomyces carlsbergensis): Characterization and propertiesBiotechnology & Bioengineering, 1981
- ISOLATION AND PROPERTIES OF PROTEASE FROM SACCHAROMYCES CARLSBERGENSISJournal of Food Biochemistry, 1980
- Lysinoalanine formation in yeast proteins isolated by alkaline methodsJournal of Agricultural and Food Chemistry, 1980
- PREPARATION OF YEAST PROTEIN ISOLATE WITH LOW NUCLEIC ACID BY SUCCINYLATIONJournal of Food Science, 1979
- Properties of intracellular ribonuclease utilized for RNA reduction in disintegrated cells ofSaccharomyces cerevisiaeBiotechnology & Bioengineering, 1977
- Chemical and nutritional modifications of sunflower proteins due to alkaline processing. Formation of amino acid crosslinks and isomerization of lysine residuesJournal of Agricultural and Food Chemistry, 1975
- Enzymatic RNA reduction in disintegrated cells ofSaccharomyces cerevisiaeBiotechnology & Bioengineering, 1974
- The Microbe as a Source of FoodAnnual Review of Microbiology, 1972