Synthesis of a Complementary DNA Probe Specific for Detecting Subterranean Clover Red Leaf Virus in Plants and Aphids

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Abstract
Two RNA species, and 1 DNA species, were isolated from subterranean clover red leaf virus (SCRLV) prepared by a modification of previously described methods. 3H-labeled c[complementary]DNA transcribed from high MW RNA of purified virus was specific for the detection of SCRLV, in that it showed no hybridization with nucleic acids from healthy [Pisum sativum] plants, or plants infected with the serologically related potato leaf roll virus, but hybridized with homologous RNA and nucleic acids from SCRLV-infected plants of 2 spp. The cDNA detected SCRLV in individuals and groups of the aphid vector, Aulacorthum solani, and the average virus content was > 170 pg/aphid. The non-vector, Myzus persicae, contained only trace amounts of SCRLV, a result confirmed by enzyme-linked immunosorbent assay.