Roles of a conserved proline in the internal fusion peptide of Ebola glycoprotein
- 15 June 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 569 (1-3) , 261-266
- https://doi.org/10.1016/j.febslet.2004.06.006
Abstract
The structural determinants underlying the functionality of viral internal fusion peptides (IFPs) are not well understood. We have compared EBOwt (GAAIGLAWIPYFGPAAE), representing the IFP of the Ebola fusion protein GP, and EBOwt (GAAIGLAWIPYFGRAAE) derived from a non-functional mutant with conserved Pro537 substituted by Arg. P537R substitution did not abrogate peptide-membrane association, but interfered with the ability to induce bilayer destabilization. Structural determinations suggest that Pro537 is required to preserve a membrane-perturbing local conformation in apolar environments.Keywords
This publication has 30 references indexed in Scilit:
- Calcium‐dependent conformational changes of membrane‐bound Ebola fusion peptide drive vesicle fusionFEBS Letters, 2002
- Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potentialPublished by Elsevier ,2002
- How proteins adapt to a membrane–water interfaceTrends in Biochemical Sciences, 2000
- Turns in transmembrane helices: determination of the minimal length of a “helical hairpin” and derivation of a fine-grained turn propensity scale 1 1Edited by F. E. CohenJournal of Molecular Biology, 1999
- A turn propensity scale for transmembrane helicesJournal of Molecular Biology, 1999
- Similar Structural Models of the Transmembrane Proteins of Ebola and Avian Sarcoma VirusesCell, 1996
- Functionalized Protein-like Structures from Conformationally Defined Synthetic Combinatorial LibrariesPublished by Elsevier ,1996
- Interaction of the HIV-1 Fusion Peptide with Phospholipid Vesicles: Different Structural Requirements for Fusion and LeakageBiochemistry, 1994
- Viral and Cellular Membrane Fusion ProteinsAnnual Review of Physiology, 1990
- Detection of a fusion peptide sequence in the transmembrane protein of human immunodeficiency virusCell, 1987