Air-injection of the corneal stroma: A SEM study

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Abstract
The corneal stroma is supposed to be a homogeneous structure both from an optical and an anatomical point of view. Intrastromal injection of glutaraldehyde as a fixative causes local swelling and opacification of the corneal tissue. Local injections of small volumes of air superficially and more deeply into the corneal stroma produce similar swellings and formation of opaque areas. SEM investigation of these areas shows the existence of stromal canals, partly pre-existing, partly caused by the internal ‘explosions’ due to the air-injection. This suggests an anatomically non-homogeneous, although optically homogeneous, cornea.

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