Identification of the magnesium, europium and lead binding sites in E. coli and lupine tRNAPhe by specific metal ion‐induced cleavages
- 30 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 243 (2) , 293-298
- https://doi.org/10.1016/0014-5793(89)80148-6
Abstract
The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydrolysis. The latter tRNA is cleaved with much lower yield in the D-loop, and interestingly, cleavage is also detected in the variable region, that is highly resistant to hydrolysis in eukaryotic tRNAs. The possible location of tight Pb, Eu and Mg binding sites in E. coli tRNAPhe is discussed on the basis of the specific hydrolysis data.Keywords
This publication has 13 references indexed in Scilit:
- Characterization of the lead(II)-induced cleavages in tRNAs in solution and effect of the Y-base removal in yeast tRNAPheBiochemistry, 1988
- Characterization of Two RNA-catalyzed RNA Cleavage ReactionsCold Spring Harbor Symposia on Quantitative Biology, 1987
- Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNABiochemistry, 1985
- Nonenzymatic hydrolysis of RNA: Pb(II)-catalyzed site specific hydrolysis of transfer RNA. The role of the tertiary folding of the polynucleotide chainInternational Journal of Quantum Chemistry, 1984
- Pb(II)-catalysed cleavage of the sugar–phosphate backbone of yeast tRNAPhe—implications for lead toxicity and self-splicing RNANature, 1983
- Transfer RNA in Solution: Selected TopicsAnnual Review of Biophysics and Bioengineering, 1980
- Further refinement of the structure of yeast tRNAPheJournal of Molecular Biology, 1978
- A crystallographic study of metal-binding to yeast phenylalanine transfer RNAJournal of Molecular Biology, 1977
- Effect of europium(III) on the thermal denaturation and cleavage of transfer ribonucleic acidsBiopolymers, 1976
- Specific cleavages of pure tRNAs by plumbous ionsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976