Modulation of Nitric Oxide Production by Tetracyclines and Chemically Modified Tetracyclines
- 1 November 1998
- journal article
- Published by SAGE Publications in Advances in Dental Research
- Vol. 12 (1) , 126-130
- https://doi.org/10.1177/08959374980120010701
Abstract
Chemically modified tetracyclines (CMTs) dose-dependently decreased inducible nitric oxide synthase (iNOS) and, consequently, nitric oxide (NO) formation by the lipopolysaccharide (LPS)-stimulated J774 line. The inhibitory effect was due to a specific reduction in the iNOS protein content in the cells, as attested by Western blot analysis and by the inhibition of iNOS mRNA accumulation. Furthermore, CMTs cause a dose-dependent increase in cell death in the J774 line mediated by the NO-independent apoptotic mechanism.Keywords
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