High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium
- 1 February 1989
- journal article
- Published by Springer Nature in Cytotechnology
- Vol. 2 (1) , 5-8
- https://doi.org/10.1007/bf00365409
Abstract
Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.Keywords
This publication has 4 references indexed in Scilit:
- High-density culture of mouse-human hybridoma cells using a new perfusion culture vessel.KAGAKU KOGAKU RONBUNSHU, 1988
- Large-Scale Cell Culture TechnologyPublished by Elsevier ,1983
- Cell Culture on Artificial Capillaries: An Approach to Tissue Growth in vitroScience, 1972
- Spin Filter Culture: The Propagation of Mammalian Cells in SuspensionScience, 1969