The Influence of Superoxide on the Production of Hypochlorous Acid by Human Neutrophils
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 12 (1) , 47-52
- https://doi.org/10.3109/10715769109145766
Abstract
Human neutrophils stimulated with opsonized zymosan promoted hypochlorous acid (HOCl)-dependent loss of monochlorodimedon. Formation of HOCl was completely inhibited by catalase, and it was also inhibited up to 70% by SOD. There was no inhibition by desferal, DTPA, mannitol or dimethylsulphoxide, which excluded the involvement of .OH. Our results indicate that generation of O2- by neutrophils enables these cells to enhance their production of HOCl. Furthermore, inhibition of neutrophil processes by SOD and catalase does not necessarily implicate .OH. We propose that O2- may potentiate oxidant damage at inflammatory sites by boosting the myeloperoxidase-dependent production of HOCl.Keywords
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