Deconstruction of GCN4/GCRE into a monomeric peptide-DNA complex
- 1 June 1995
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 2 (6) , 450-457
- https://doi.org/10.1038/nsb0695-450
Abstract
Here we describe a system that enables short peptides to bind DNA sequence-specifically. Linking the peptide covalently to DNA through a disulphide bond eliminates the unfavourable energetic cost of diffusion and thus potentiates the peptide-DNA interaction. By this approach we have deconstructed the GCN4/DNA complex into its elemental DNA recognition units. We find that the GCN4 basic region contacts the two half-sites with very different affinities and propose that this thermodynamic asymmetry plays a role in differential regulation of gene expression. Specific binding of the peptide to DNA stabilizes the disulphide bond toward reduction suggesting a novel approach to the discovery of new DNA-binding specificities.Keywords
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