Sevoflurane Is Biotransformed by Guinea Pig Liver Slices but Causes Minimal Cytotoxicity

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Abstract
Guinea pig liver slices were used to evaluate the biotransformation and hepatotoxic potential of sevoflurane. Precision-cut liver slices (250–300 μm thick) were incubated in sealed roller vials in buffer at 37°C under 95% O2. Sevoflurane was added to produce 0.9 or 2.1 mM medium concentrations. After incubation (6–24 h), the intracellular K+ content and protein synthesis were determined, along with the defluorination of Sevoflurane. Isoflurane was included for comparative purposes. Sevoflurane (2.1 mM) and isoflurane (2.3 mM) had no effect on slice K+ content, but both anesthetics depressed protein synthesis. The biotransformation of Sevoflurane was maximal at 95% O2, with threefold more F− produced from Sevoflurane than isoflurane. Sevoflurane appears to have a minimal effect on the guinea pig liver slices, which is consistent with in vivo studies in which minimal or no hepatotoxicity has been observed.

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