Neutralization of divergent human immunodeficiency virus type 1 variants and primary isolates by IAM-41-2F5, an anti-gp41 human monoclonal antibody.
- 12 April 1994
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (8) , 3348-3352
- https://doi.org/10.1073/pnas.91.8.3348
Abstract
The antiviral characteristics of monoclonal antibody IAM-41-2F5 (2F5) were determined in cell culture. The antibody had been previously shown to bind a specific sequence, ELDKWA, within the external domain of the gp41 envelope glycoprotein human immunodeficiency virus type 1 (HIV-1). Selection by 2F5 of recombinant phage from an epitope library confirmed the identification of the antibody's binding determinant. The antibody was found to be capable of neutralizing a broad range of lymphoid cell culture-adapted HIV-1 variants as well as HIV-1 primary isolates. Sequence analysis of the latter showed that neutralization was related to the presence of the antibody binding site. From kinetic measurements using an epitope-containing peptide or gp41, the half-time of dissociation for 2F5 was determined to be 122 min for the peptide and 156 min for gp41. The region of gp41 expressing this sequence exhibits greater conservation among HIV-1 isolates than do the variable domains of gp120.Keywords
This publication has 20 references indexed in Scilit:
- Discontinuous, conserved neutralization epitopes overlapping the CD4-binding region of human immunodeficiency virus type 1 gp120 envelope glycoproteinJournal of Virology, 1992
- Production of Human Monoclonal Antibodies Specific for Conformational and Linear Non-V3 Epitopes of gp120AIDS Research and Human Retroviruses, 1992
- Vertical transmission of human immunodeficiency virus (HIV) infection. Reactivity of maternal sera with glycoprotein 120 and 41 peptides from HIV type 1.Journal of Clinical Investigation, 1992
- Prevention of HIV-1 infection in chimpanzees by gpl20 V3 domain-specific monoclonal antibodyNature, 1992
- Identification of human neutralization-inducing regions of the human immunodeficiency virus type 1 envelope glycoproteins.Proceedings of the National Academy of Sciences, 1992
- Generation and characterization of a human monoclonal antibody that neutralizes diverse HIV-1 isolates in vitroAIDS, 1992
- Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical systemJournal of Immunological Methods, 1991
- An IgG human monoclonal antibody that reacts with HIV-1/GP120, inhibits virus binding to cells, and neutralizes infection.1991
- Immunization of chimpanzees confers protection against challenge with human immunodeficiency virus.Proceedings of the National Academy of Sciences, 1991
- A microtiter cell-culture assay for the determination of anti-human immunodeficiency virus neutralizing antibody activityJournal of Virological Methods, 1988