Persistent Theiler's Virus in Ependymoma Tissue Culture and the Problem of Virus-Like Bodies Seen by Electron Microscopy2

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Abstract
Theiler's GDVII virus persistently infected mouse ependymoma cells in tissue culture; infectious virus might persist for longer than a year, or it might spontaneously disappear after several months. Electron-dense oval bodies approximately 85 mµ in diameter were found at the surface of cells infected only for 6 days and at a time when the viral titer was maximal. The nature of these bodies is unknown. Uninfected ependymoma cells of the strain studied contained electron-dense cytoplasmic bodies of another type. They were approximately 65 mµ in diameter, double-walled (type A Bernhard), and appeared to originate from the walls of cytoplasmic vacuoles. These were similar to bodies in various murine tumors described by other investigators.