SPERM-IMMOBILIZING MONOCLONAL ANTIBODY TO HUMAN SEMINAL PLASMA ANTIGENS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 42  (3) , 458-462
Abstract
Rat spleen cells immunized to human azoospermic semen (a mixture of seminal plasma components) and mouse myeloma cells (P3/X63 Ag8U1; P3U1) were successfully fused with polyethylene glycol (PEG 1500) and 19 of 89 fused cell cultures produced sperm-immobilizing antibody. The cells that produced antibody indicating the highest sperm-immobilizing activity were distributed into wells for further recloning and 10 clones producing sperm-immobilizing antibody were established. The clone (1C4) producing the highest antibody titer was found to produce a large amount of IgG in culture supernatants and to contain a mixture of rat and mouse chromosomes. The monoclonal antibody was produced to the human seminal plasma antigen No. 7 which is common to human milk protein. Using this hybridoma which produced a large amount of monoclonal sperm-immobilizing antibody, a new method could be developed for purifying human seminal plasma antigen by immunoaffinity chromatography with bound antibody from the hybridoma. Implications in sterility are discussed.

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