Biosensing with coated-wire electrodes. Part 2. Urea sensors

Publisher Website
Google Scholar
Abstract
Enzyme electrodes are described for the determination of urea based on an ammonium ion-selective coated-wire electrode. The electrodes consisted of homogeneous membranes of poly(vinyl chloride)(PVC) or silicone rubber containing both the electroactive species and immobilised enzyme. Those sensors in which PVC was utilised as the membrane proved to be the most useful and for a 0.2 mm thick membrane a super-Nernstian calibration was obtained with a mean slope of 61 ± 1 mV decade–1 under static conditions of measurement. The lifetime of the electrodes was approximately 10 d. The electrode could be used in a miniaturised, custom-designed flow cell for automated analysis. In this mode the slope of the calibration obtained was 67 ± 2 mV decade–1.

This publication has 0 references indexed in Scilit: