Partition of Cell Particles in Three-phase Systems.

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Abstract
Three-phase systems of extraction consisting of water and 3 polymers, dextran, Ficoll and polyethylene glycol, were used for partition of some cell particles. Particles of biological origin are stable in these systems due to the high water content, 60-75%. The partition behavior of a particle is strongly affected by introducing charged polymers in the 3-phase system. By varying the amount of charged polymer the affinity of particles for the different phases can successively be changed. In this way Chlorella pyrenoidosa, Saccharomyces cerevisiae and subchloroplast particles were shown to consist of more than 1 fraction. These fractions are separated from each other by a single partition step. By introducing hydrophobic groups to one of the polymers, the partition of cell particles can in some cases be affected. Subchloroplast particles and mitochondria were resolved into several fractions, differing in hydrophobicity, with such 3-phase systems.

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