Improvement on the competitive binding assay for the measurement of cyclic AMP by using ammonium sulphate precipitation
- 1 August 1987
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 245 (3) , 923-924
- https://doi.org/10.1042/bj2450923
Abstract
The protein-binding assay developed by Brown, Albano, Ekins, Sgherzi & Tampion [(1971) Biochem. J. 121, 561-562] and Brown, Ekins & Albano [(1972) Adv. Cyclic Nucleotide Res. 2, 25-40] was modified by using precipitation with (NH4)2SO4 of the protein-cyclic AMP complex instead of adsorption of the free nucleotide on charcoal. The half-life of the protein-cyclic AMP complex obtained in the presence of charcoal was lower than that of the (NH4)2SO4-precipitated complex. In consequence, owing to the great stability of the precipitated protein-cyclic AMP complex, this method allows more accurate and reproducible determinations.This publication has 1 reference indexed in Scilit: