Inhibition of LTB4 binding to human neutrophils by nordihydroguaiaretic acid

Abstract
Nordihydroguaiaretic acid (NDGA) was investigated for its ability to interact with leukotriene B4 receptors on human polymorphonuclear leukocytes (hPMNs).3H-LTB4 binding to specific receptors was reduced in a dose-dependent manner with maximal reduction at 100 μM NDGA and an IC50 of about 50 μM. Binding of another inflammatory stimulus, N-formyl-norleucyl-leucyl-phenylalanine (FNLP) was not affected by similar treatment. Chemotaxis and enzyme release stimulated by LTB4 and oligopeptide were inhibited by NDGA. In addition, LTB4-triggered inflammationin vivo in mice was inhibited by systemic administration of NDGA. These data suggest that LTB4 receptor antagonism may contribute to inhibition of inflammation by NDGA.