Rapid Fish Species Identification by Agarose Gel Isoelectric Focusing of Sarcoplasmic Proteins
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 64 (1) , 38-43
- https://doi.org/10.1093/jaoac/64.1.38
Abstract
A rapid method is described for fish species identification by agarose gel isoelectric focusing (AGIEF). The AGIEF method can be completed in less than 2 h and gives reproducible species-specific sarcoplasmic protein patterns. Protein patterns are similar using either centrifuged tissue fluid or muscle tissue as the sample. One species, monkfish (Lophius americanus), has a polymorphic protein pattern. A predominant pattern was found in 66.7% of the individuals; 2 variant patterns were equally distributed among the remaining 33.3%. AGIEF offers a more rapid, less expensive alternative to the current AOAC official first action method for fish species identification based on polyacrylamide gel isoelectric focusing.This publication has 5 references indexed in Scilit:
- Fish Species Identification By Thin Layer Polyacrylamide Gel Isoelectric Focusing: Collaborative StudyJournal of AOAC INTERNATIONAL, 1980
- Direct tissue isoelectric focusing in agaroseJournal of Immunological Methods, 1979
- Fish Species Identification by Thin Layer Isoelectric FocusingJournal of AOAC INTERNATIONAL, 1979
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- Isoelectric points and molecular weights of proteinsJournal of Chromatography A, 1976