Plasma Catecholamines Assay: Comparison Between Fluorimetric and Radioenzymatic Methods

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Abstract
Plasma catecholamines were simultaneously measured in duplicate plasma samples by the fluorimetric method of Renzini et al. and by the radioenzymatic method of Da Prada and Zurcher. The correlation of noradrenaline and adrenaline [norepinephrine and epinephrine] plasma concentrations determined by the 2 methods were, respectively, r = 0.95 (P < 0.001) and r = 0.75 (P < 0.01). The fluorimetric method was less sensitive, but more economical and less time-consuming than the radioenzymatic method. The fluorimetric method is still of value for measuring plasma catecholamines in man.

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