Dissociation of Mammalian Polyribosomes into Subunits by Puromycin
- 1 February 1971
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (2) , 390-394
- https://doi.org/10.1073/pnas.68.2.390
Abstract
Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0°C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA. The polysomes were then heated to 37°C, when they dissociated completely into subunits. Similar treatment without puromycin resulted in only partial dissociation.Keywords
This publication has 13 references indexed in Scilit:
- Structure and function of mammalian ribosomes: I. Isolation and characterization of active liver ribosomal subunitsJournal of Molecular Biology, 1970
- CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONSThe Journal of cell biology, 1970
- The effect of incubation with puromycin on the dissociation of rat liver ribosomes into active subunitsBiochemical and Biophysical Research Communications, 1969
- Purification and properties of dog pancreas ribosomes and subunitsBiochemistry, 1969
- Active hybrid 80 s particles formed from subunits of rat, rabbit and protozoan (Tetrahymena pyriformis) ribosomesJournal of Molecular Biology, 1969
- RELEASE OF TRANSFER RNA DURING PEPTIDE CHAIN ELONGATIONProceedings of the National Academy of Sciences, 1969
- Ribosome‐Catalyzed Peptidyl TransferEuropean Journal of Biochemistry, 1968
- Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimentable and ribosome fractions of rat liver after a single injection of labeled orotic acidBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Studies on free and membrane-bound ribosomes in rat liverJournal of Molecular Biology, 1967
- Factors influencing the puromycin-induced release of protein from liver ribosomesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966