High Pressure Liquid Chromatographic Determination of Polynuclear Aromatic Hydrocarbons in Oysters
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 62 (1) , 29-35
- https://doi.org/10.1093/jaoac/62.1.29
Abstract
A high pressure liquid chromatographic (HPLC) procedure is described for determining 13 polynuclear aromatic hydrocarbon (PNA) compounds in oysters at the 2 ppb level. These compounds are extracted from shellfish with acetonitrile and partitioned into petroleum ether; the petroleum ether is removed and the residue is saponified. The aromatic compounds are isolated by passing the saponified residue through silica gel and further purified and fractionated by μStyragel gel permeation chromatography. The individual PNAs are then quantitatively determined by using a reverse phase HPLC column coupled to fluorescence, spectrophotometric, and 254 nm absorbance detectors in series. Recoveries from spiked samples generally were >80%.This publication has 1 reference indexed in Scilit:
- Gas chromatographic analysis of polycyclic aromatic hydrocarbons in soot samplesEnvironmental Science & Technology, 1967