Radioimmunoassay for Antibodies to Cytoplasmic Ribosomes in Human Serum

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Abstract
A radiommunoassay for the detection of antibodies in human serum to tritium-labeled [human cervical carcinoma] HeLa cell cytoplasmic ribosomes was developed with the use of Macaloid for the inhibition of endogenous ribonuclease activity. Antibodies were observed in the serum of patients wiht systemic lupus erythematosus in high incidence and titer. Patients with rheumatoid arthritis and chronic active hepatitis manifested a lower incidence and titer of antibodies to ribosomes, whereas sera from normal individuals and from patients with sarcoidosis, chronic glomerulonephritis and malignant tumors showed no significant reactivity with cytoplasmic ribosomes. Maximum inhibition of the reaction was achieved with unlabeled HeLa cell ribosomes or rat liver ribosomes and partial inhibition by purified ribosomal RNA.