Cardiac troponin I, isolated from‐bovine heart, contains two adjacent phosphoserines
Open Access
- 1 September 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 176 (2) , 335-342
- https://doi.org/10.1111/j.1432-1033.1988.tb14286.x
Abstract
Bovine cardiac troponin containing approximately 3 mol P/mol protein could be separated into its subunits without loss of phosphate. Troponin I and troponin T each contain about 1.5 mol P/mol protein. In troponin I two phosphorylated serine residues could be localized in the N‐terminal region by conversion of phosphoserine to S‐ethylcysteine. They are located in adjacent positions in the following sequence:‐Arg‐Arg‐Ser(P)‐Ser(P)‐Ala‐Asn‐Tyr‐Tyr‐Arg‐Ala‐Tyr‐Ala‐Thr‐Glu‐Pro‐His‐Ala‐Lys. This sequence shows that the first phosphoserine residue in bovine cardiac troponin I occupies a homologous position to phosphoserine‐20 of rabbit cardiac troponin I.This publication has 21 references indexed in Scilit:
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