ROLE OF CYCLIC-AMP IN REGULATION OF CHLORIDE SECRETION BY CANINE TRACHEAL MUCOSA

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Abstract
CAMP mediates the action of many hormones, and it affects ion transport in several different epithelia. The effect of the analog dibutyryl cAMP was tested in canine tracheal epithelium in vitro under short-circuit conditions. This agent raised short-circuit current and electrical conductance. Dibutyryl cAMP at 1 mM concentration added to the submucosal bath increased net Cl secretion from 1.74 .+-. 0.40 to 2.72 .+-. 0.34 .mu.eq/cm2 .cntdot. h (mean .+-. SE, n = 6). Net Na absorption was slightly reduced. Dibutyryl cAMP and theophylline decreased mannitol flux, suggesting that cAMP acts on Cl transport by increasing its flow through cellular rather than through paracellular pathways. Epinephrine, which also stimulates Cl secretion, causes significant elevation in cAMP in epithelial cells scraped from tracheal mucosa. Cellular concentrations of cAMP may play an important role in the regulation of Cl transport by tracheal mucosa.

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