ANTIBODY PRODUCTION STUDIED BY MEANS OF LHG ASSAY .I. SPLENIC RESPONSE OF CBA MICE TO SHEEP ERYTHROCYTES

  • 1 January 1966
    • journal article
    • research article
    • Vol. 11  (6) , 603-+
Abstract
By means of a modified plaque assay the numbers of cells producing 19S antibody (direct PFC [plaque forming cells]) and 7S antibody (developed PFC) have been studied separately. Significan and somewhat surprising differences in the responses of the 2 kinds of antibody-producing cell have been noted. It is clear that the route of injection of the antigen is of importance in determining the response in the spleen. The response of both types of PFC was found to be biphasic. The total increase in spleen cell number after immunization was not readily accounted for by the increase in specific PFC.