A Modified Technique for Combination of Cytochemistry and Radioautography of Haemic Cells
- 1 January 1977
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Haematology
- Vol. 18 (1) , 54-60
- https://doi.org/10.1111/j.1600-0609.1977.tb01477.x
Abstract
Cultured mouse bone marrow and human blood cells were labeled with 3H-thymidine, smeared, and stained, using peroxidase reagents or the May-Grunwald Giemsa procedure. The slides were dipped in a 12% solution of polyvinylchloride, bleached by a photographic fixer and processed for liquid emulsion radioautography. This method yielded technically very satisfactory radioautographs of pre-stained smears.Keywords
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