Abstract
Twenty-three microbial cultures were surveyed for their ability to utilize orthophosphite phosphorus for heterotrophic growth and to accumulate the oxidation product, orthophosphate, in the growth medium. Of these, 14 utilized orthophosphite for growth, but only Pseudomonas fluorescens strain 195 accumulated orthophosphate. A study of orthophosphate accumulation by this pseudomonad revealed that the accumulation occurred in a linear fashion after an initial 24-hr incubation period and that the rate decreased when most of the carbohydrate had been removed from the medium. All variations tried in the growth medium resulted in reduced accumulation.

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