Methionine biosynthesis and S-adenosylmethionine degradation during an induced morphogenesis of Candida albicans

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Abstract
Seven strains of Candida albicans were used in studies designed to delineate altered biochemical activity during an induced yeast to filamentous morphogenesis. Four of the strains studied underwent a rapid (1–2 h) yeast to filamentous transformation in a defined medium with glycine as the main nitrogen source; three other strains of C. albicans remained yeast-like under the same growth conditions. Cell-free extracts, prepared from cells harvested after various time intervals of growth in glycine medium, were used to measure enzymic capacity to form methionine, via the enzyme S-adenosylmethionine (or methylmethionine) homocysteine methyltransferase, and degrade S-adenosylmethionine. Increased enzymic capacity to synthesize methionine (with S-adenosylmethionine as methyl donor) and degrade S-adenosylmethionine appeared to correlate best with a yeast to filamentous transformation of C. albicans. Intracellular S-adenosylmethionine content also decreased with time, in all seven strains, when the cells were cultured in the glycine medium. S-adenosylmethionine may play a key role in the biochemical alterations that occur during the morphogenesis of C. albicans.

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