Sterol content and enzyme defects of nystatin-resistant mutants of Neurospora crassa
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 165 (3) , 305-308
- https://doi.org/10.1007/bf00332531
Abstract
Non-saponifiable cell extracts of wild type and sterol mutants of N. crassa were analysed by means of gas-liquid chromatography. The wild-type contained ergosterol and episterol in a 10:1 ratio. None of the mutants was able to synthesize ergosterol. Three of the mutants carry single recessive gene mutations causing blocks in the terminal steps of ergosterol biosynthesis: erg-1 has an inactive Δ 8 →Δ 7 isomerase, erg-2 has an inactive 24(28) hydrogenase, and erg-4 has an inactive C-24 methyl transferase. Some of the mutants accumulated novel sterols as a result of their enzyme defects. The genes erg-1 and erg-2 were mapped close to inl on the right arm of chromosome V.This publication has 15 references indexed in Scilit:
- Biosynthesis of terpenes and steroids. Part X. The sterols of some yeast mutants doubly defective in ergosterol biosynthesisJournal of the Chemical Society, Perkin Transactions 1, 1975
- Detection of the ergosterol and episterol isomers lichesterol and fecosterol in nystatin-resistant mutants of Neurospora crassaBiochemical Genetics, 1974
- Studies on the accumulation of ergosterol and 24(28)-dehydroergosterol in 3 strains of Saccharomyces cerevisiae.1974
- The efficacy of various mutagens and polyene antibiotics for the induction and isolation of sterol mutants of Neurospora crassaMolecular Genetics and Genomics, 1974
- Biosynthetic implications of the sterol content of ergosterol-deficient mutants of yeastJournal of the Chemical Society, Chemical Communications, 1974
- Sterol mutants of Neurospora crassa: Their isolation, growth characteristics and resistance to polyene antibioticsMolecular Genetics and Genomics, 1973
- Biosynthesis of terpenes and steroidsBioorganic Chemistry, 1973
- Investigations on the biosynthesis of steroids and terpenoids. Part VI. The sterols of yeastJournal of the Chemical Society, Perkin Transactions 1, 1972
- Relation between structure and retention time of sterols in gas chromatograpphyAnalytical Chemistry, 1971
- Distribution of Lysine Pathways Among Fungi: Evolutionary ImplicationsThe American Naturalist, 1964