Use of a cellular potyadenylation signal by viral transcripts in polyoma virus transformed cells
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (15) , 4515-4524
- https://doi.org/10.1093/nar/10.15.4515
Abstract
The DNA sequences at and around the junctions between viral and cellular DNA in the polyoma virus transformed mouse cell line, TS-A-3T3, have been determined. No common sequence specificity or structural features at the joins have been observed. The sequence indicates that the 94K truncated large T antigen found in TS-A-3T3 cells is a hybrid protein in which the carboxy-terminal 19 amino acids are encoded by adjacent host sequences. Moreover, the three early region transcripts initiated in viral sequences are also hybrid in nature and appear to utilize a host polyadenylation signal associated with the hexanucleotide, AATAAA, found 100 bp beyond a viral-host join.Keywords
This publication has 19 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Characterization of polyoma viral DNA sequences in polyoma-induced hamster tumor cell lines.Journal of Biological Chemistry, 1980
- The Polyoma virus 100K large T-antigen is not required for the maintenance of transformationVirology, 1980
- The ovalbumin gene - sequence of putative control regionsNucleic Acids Research, 1980
- Coding potential and regulatory signals of the polyoma virus genomeNature, 1980
- Polyoma virus-specific 55K protein isolated from plasma membrane of productively infected cells is virus-codedand important for cell transformationVirology, 1979
- Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinatesNucleic Acids Research, 1979
- Computer analysis of nucleic acid regulatory sequences.Proceedings of the National Academy of Sciences, 1977
- Sequence at the 3′ end of globin mRNA shows homology with immunoglobulin light chain mRNANature, 1974
- Polyma DNA: A Physical MapProceedings of the National Academy of Sciences, 1974