The ubiquitin-specific protease USP28 is required for MYC stability
- 10 June 2007
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 9 (7) , 765-774
- https://doi.org/10.1038/ncb1601
Abstract
The MYC proto-oncogene encodes a transcription factor that has been implicated in the genesis of many human tumours. Here, we used a bar-code short hairpin RNA (shRNA) screen to identify multiple genes that are required for MYC function. One of these genes encodes USP28, an ubiquitin-specific protease. USP28 is required for MYC stability in human tumour cells. USP28 binds to MYC through an interaction with FBW7α, an F-box protein that is part of an SCF-type ubiquitin ligase. Therefore, it stabilizes MYC in the nucleus, but not in the nucleolus, where MYC is degraded by FBW7γ. High expression levels of USP28 are found in colon and breast carcinomas, and stabilization of MYC by USP28 is essential for tumour-cell proliferation.Keywords
This publication has 49 references indexed in Scilit:
- Aberrant stabilization of c-Myc protein in some lymphoblastic leukemiasLeukemia, 2006
- Ubiquitylation of Cyclin E Requires the Sequential Function of SCF Complexes Containing Distinct hCdc4 IsoformsMolecular Cell, 2006
- Specific Requirement for Bax, Not Bak, in Myc-induced Apoptosis and Tumor Suppression in VivoPublished by Elsevier ,2006
- A loss-of-function RNA interference screen for molecular targets in cancerNature, 2006
- Evasion of the p53 tumour surveillance network by tumour-derived MYC mutantsNature, 2005
- MYC inactivation uncovers pluripotent differentiation and tumour dormancy in hepatocellular cancerNature, 2004
- The β-Catenin/TCF-4 Complex Imposes a Crypt Progenitor Phenotype on Colorectal Cancer CellsCell, 2002
- Phosphorylation-Dependent Ubiquitination of Cyclin E by the SCF Fbw7 Ubiquitin LigaseScience, 2001
- Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell lineNature, 2001
- Induction of apoptosis in fibroblasts by c-myc proteinCell, 1992