RAPID METHODS FOR THE DETERMINATION OF TOTAL HOP BITTER SUBSTANCES (ISO-COMPOUNDS) IN BEER

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Abstract
Rapid methods for the determination of the sum of isohumulone, isocohumulone and isoadhumulone in bear have been developed. Rapid Method I consists essentially of the extraction of the iso-compounds from acidified beer with a measured quantity of 2,2,4-trimethylpentane followed by spectrophotometric measurement of the concentration of iso-compounds in an aliquot of the extract. The apparent values given by this method are approximately 30% higher than countercurrent distribution values, owing to background absorption, but can be reduced to real values by means of a regression equation obtained from the relationship between the values obtained by the countercurrent and rapid methods. This procedure should be adequate for most routine purposes. Where higher accuracy is desirable, Rapid Method II may be used. This is basically the same as Method I, but employs a phase separation to eliminate most of the substances which contribute to background absorption. Values obtained by Method II and by countercurrent distribution are in close agreement.

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