Enrichment of hapten‐specific helper T cells using anti‐immunoglobulin combining site antibodies

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Abstract
Spleen T cells from phosphorylcholine (PC)-primed BALB/c mice were indirectly stained with affinity column-purified heterologous rabbit anti-TEPC 15 (T15) antibodies. These antibodies were shown to be directed towards antigenic determinants in or near the combining site of T15 in that their interaction with the myeloma protein was more than 95% inhibit able by PC. The stained cells were sorted into “bright” and “dull” fractions using a fluorescence-activated cell sorter. Sorted populations were then assessed for enrichment and depletion of PC-specific helper activity using an in vivo adoptive transfer assay. The results indicate that the staining procedure employed enabled the specific enrichment of PC helper T cells and therefore suggest that these T cells bear combining site structures similar to that of the myeloma protein T15.

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