Micellar High-Performance Liquid Chromatographic Separation of Serum Bilirubin Species with Direct Sample Injection

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Abstract
A rapid reversed-phase high performance liquid chromatographic method is described using a C8 column and a mobile phase containing sodium dodecyl sulfate (SDS) as a modifier to separate the four bilirubin species present in serum: unconjugated, monoconjugated, diconjugated and biliprotein. The results show that using SDS as a mobile phase modifier improves separation efficiency and increases sample solubility. This simple HPLC procedure allows direct sample injection and makes it possible to quantitatively determine bilirubin species in biological fluids.