Synthesis of RNA-Polyadenylic Acid by Isolated Brain Nuclei
- 14 September 1973
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 181 (4104) , 1064-1065
- https://doi.org/10.1126/science.181.4104.1064
Abstract
Nuclei, isolated from mouse brain tissue at various stages of postnatal development and incubated under cell-free conditions, synthesized RNA molecules that were associated with polyadenylic acid [poly(A)]. The RNA synthesized by these nuclei was similar to the poly(A)-associated products described for intact eukaryotic cells. The brain nuclei synthesized a similar proportion of RNA-poly(A) in the presence either of Mg 2+ or of Mn 2+ with (NH 4 ) 2 SO 4 . The RNA from neonatal brain nuclei appeared to have a greater proportion of poly(A)-containing RNA than nuclear products obtained from more mature neural tissue.Keywords
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